rabbit anti-human ifnar2  (PBL Biomedical Laboratories)

Journal: The Journal of Reproduction and Development

Article Title: Pregnancy-associated changes of peroxisome proliferator-activated receptor delta (PPARD) and cytochrome P450 family 21 subfamily A member 2 (CYP21A2) expression in the bovine corpus luteum

doi: 10.1262/jrd.2019-132

Figure Lengend Snippet: Primers used in real-time PCR

Article Snippet: The 5 µm-thick sections from the paraffin-embedded luteal tissue were incubated at room temperature (20˚C) with rabbit polyclonal anti-human PPARD antibody (#ARP38765_T100, Aviva Systems Biology, San Diego, CA, USA; 1:50), rabbit polyclonal anti-human CYP21A2 antibody (#ARP60144_P050, Aviva Systems Biology; 1:25), rabbit polyclonal anti-human IFNαR1 antibody (#bs-4116R, Bioss, Woburn, MA, USA; 1:50) or rabbit polyclonal anti-human IFNαR2 antibody (#bs-7022R, Bioss; 1:50) for 12 h. The signals were detected using anti-rabbit IgG-Biotin conjugate (Sigma-Aldrich, LLC, St Louis, MO, USA; 1:100) for 1 h, and then counterstained with hematoxylin.

Techniques: Sequencing

Journal: The Journal of Reproduction and Development

Article Title: Pregnancy-associated changes of peroxisome proliferator-activated receptor delta (PPARD) and cytochrome P450 family 21 subfamily A member 2 (CYP21A2) expression in the bovine corpus luteum

doi: 10.1262/jrd.2019-132

Figure Lengend Snippet: Changes in the relative amount of mRNA for IFNαR1 and IFNαR2 in the bovine corpus luteum (CL) on day 18 of pregnant cows (P18) and on day 15 of non-pregnant cows (NP15). Data represent means ± SEM for four cows per stage and have been expressed as relative ratios of the mRNAs to 18S ribosomal RNA ( RN18S1 ). Localization of IFNαR1 and IFNαR2 in bovine CL obtained on P18 and NP15. Intensive immunoreactivity was observed in the luteal cells (black arrowheads), endothelial cells (red arrowheads), and immune cells (yellow arrowheads) obtained from the CL of both pregnant and nonpregnant cows. No positive immunoreactivity was observed in the negative control. Scale bar = 50 µm.

Article Snippet: The 5 µm-thick sections from the paraffin-embedded luteal tissue were incubated at room temperature (20˚C) with rabbit polyclonal anti-human PPARD antibody (#ARP38765_T100, Aviva Systems Biology, San Diego, CA, USA; 1:50), rabbit polyclonal anti-human CYP21A2 antibody (#ARP60144_P050, Aviva Systems Biology; 1:25), rabbit polyclonal anti-human IFNαR1 antibody (#bs-4116R, Bioss, Woburn, MA, USA; 1:50) or rabbit polyclonal anti-human IFNαR2 antibody (#bs-7022R, Bioss; 1:50) for 12 h. The signals were detected using anti-rabbit IgG-Biotin conjugate (Sigma-Aldrich, LLC, St Louis, MO, USA; 1:100) for 1 h, and then counterstained with hematoxylin.

Techniques: Negative Control

Journal: Respiratory Research

Article Title: Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD.

doi: 10.1186/s12931-024-02952-6

Figure Lengend Snippet: Main immunohistochemical findings in the study groups

Article Snippet: Briefly, formalin-fixed paraffin embedded lung sections were treated for 60 min with primary antibodies rabbit anti-human IFNAR1 and IFNAR2 (GTX54322 and GTX54289 from GeneTex, Irvine CA, USA), MDA-5 (700360 from Invitrogen, Carlsbad CA, USA) and murine anti-human IRF-3 (sc-376455 from Santa Cruz Dallas TX, USA).

Techniques: Immunohistochemical staining

Journal: Respiratory Research

Article Title: Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD.

doi: 10.1186/s12931-024-02952-6

Figure Lengend Snippet: Additional immunohistochemical findings (intensity score in alveolar walls and bronchiolar epithelium)

Article Snippet: Briefly, formalin-fixed paraffin embedded lung sections were treated for 60 min with primary antibodies rabbit anti-human IFNAR1 and IFNAR2 (GTX54322 and GTX54289 from GeneTex, Irvine CA, USA), MDA-5 (700360 from Invitrogen, Carlsbad CA, USA) and murine anti-human IRF-3 (sc-376455 from Santa Cruz Dallas TX, USA).

Techniques: Immunohistochemical staining

Journal: International Journal of Oncology

Article Title: Stronger growth-inhibitory effect of interferon (IFN)-β compared to IFN-α is mediated by IFN signaling pathway in hepatocellular carcinoma cells

doi: 10.3892/ijo.30.1.201

Figure Lengend Snippet: Figure 6. Neutralizing assays with anti-human IFNAR2 antibody on PLC/ PRF/5 cells. (A) Blockade of IFN signaling by the antibody. The cells were incubated with or without 1:5000 diluted anti-IFNAR2 antibody for 24 h, then they were induced with 2.5 ng/ml either of IFN-· or IFN-ß. Induction of pSTAT1 was blocked for both IFNs, but less for IFN-ß, with the antibody. The expression value of each protein band was calculated relative to that of actin. Data are representative of similar results of repeat experiments. (B) The antiproliferative effect of the IFNs (2.5 ng/ml) was examined in the presence of serial dilutions of anti-IFNAR2 antibody as described in Materials and methods. The antibody significantly reduced the antiproliferative effect of IFNs (*p<0.05 vs control without the antibody by Dunnett's test). Suppression of the effects of IFN-ß required more antibody than that of IFN-·.

Article Snippet: Specific rabbit anti-human IFNAR2 (developed by Otsuka Pharmaceutical using recombinant human IFNAR2 as described in ref. 14), STAT1, pSTAT1 (Tyr701) (Cell Signaling Technology, Beverly, MA), STAT2, pSTAT2 (Tyr689) (Upstate Biotechnology, Lake Placid, NY), STAT3, pSTAT3 (Tyr705) (Cell Signaling Technology), actin (Sigma, St. Louis, MO), and donkey anti-rabbit coupled to horseradish peroxidase (Amersham Biosciences, Buckinghamshire, UK) antibodies were used.

Techniques: Incubation, Expressing, Control